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KMID : 0620920000320040243
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Experimental & Molecular Medicine
2000 Volume.32 No. 4 p.243 ~ p.245
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Analysis of trimethylsilyl derivatization products of phosphatidylethanol by gas chromatography-mass spectrometry
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Joong Soo Han/Chang Suek Yon
Joong Soo Han
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Abstract
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For the detection of rare phospholipid, phosphatidylethanol (PEt), GC-MS analysis method was adopted for the detection of derivatization products of PEt by N,O-bis (trimethylsilyl) trifluroacetamide (BSTFA). A re-structured molecule derived from PEt, ethyl bis (trimethylsilyl)-phosphate was found from search of Wiley database. This molecule can be used as a marker for PEt analysis. The molecular formula was C8H23O4PSi2 and weight of the formula was 270.09.
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KEYWORD
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GC-MS, phosphatidylethanol, trimethylsilyl derivative, BSTFA,
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